Effect of curcuminoids on DPPH radical scavenging

Scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals by 3b, cysteine, and ascorbic acid (2–200 µM).

Raw data including the absolute values (absorbance subtract ethanol blank) and normalized data (% of control) of all individual experiments was uploaded.

The methods and results were published in Rao et al., Biochem Pharmacol. 2022 Sep:203:115202. doi: 10.1016/j.bcp.2022.115202 

Vehicle (ethanol), compound 3b, cysteine hydrochloride (reference compound, Merck) or ascorbic acid (reference compound, Caesar & Loretz GmbH, Hilden, Germany) were incubated with 2,2-diphenyl-1-picrylhydrazyl (Merck; 50 µM) under shaking in the dark. After 30 min, the absorbance was measured at 520 nm using a Multiskan Spectrum Microplate Reader (Thermo Fisher Scientific).