NanoBioRS
Published December 1, 2025 | Version 1.0.0
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Data supporting article Thiolated Hyaluronic Acid: A Gateway for Targeted Killing of Staphylococcus aureus on ´the Race for Surface´ Colonization

Authors/Creators

  • 1. ROR icon Universität Innsbruck

Description

Description

Abstract: Hyaluronic acid (HA) is degraded by Staphylococcal hyaluronate lyase (Hysa) and mammalian hyaluronidase (Hyal). We aim to use thiolated HA (HAMS) as a targeted gateway for Staphylococcus aureus killing while enhancing our previous M23 endolysin–polyphosphate (M23-PP NPs) enzyme-responsive nanoparticle formulation.

Synthesis of HAMS and characterization for nuclear magnetic resonance, solubility, thiol content, pKa, and degradation by Hysa and Hyal is presented. Nanoparticles prepared via ionotropic gelation between M23-PP NPs and either HAMS or HA, yield to M23-PP/HAMS or M23-PP/HA NPs, respectively. Their characterization includes size, zeta potential, morphology, release profiles, safety, targeted release, and efficacy.

HAMS with a thiol content of 250.18 ± 90.32 µmol/g, solubility of 50.99±0.02 mg/mL, exhibits pKa values of 3.2, 4.2, and 8.8. This thiolated polymer irreversibly inhibits Hyal activity, without affecting Hysa. M23-PP/HAMS NPs (265 ± 47 nm, -25 mV) maintain their integrity for seven days at 37 °C, and HAMS coating prevents nonspecific degradation by Hyal, as confirmed by release studies.

In a co-culture ‘race for the surface’ experiment with MC3T3 osteoblasts and S. aureus ATCC 25923, M23-PP/HAMS NPs produce 8-log bacterial killing while promoting in vitro wound healing.

Our findings are pivotal to the development of new enzyme-responsive excipients switchable by S. aureus.

Context and methodology

  • This dataset  was created on the frame of NanoBioRS project European Union's Horizon 2020 Research and Innovation Programme under Marie Skłodowska-Curie IF [NanoBioRS-101025065].
  • This dataset corresponds to raw data underlying the publication Thiolated Hyaluronic Acid: A Gateway for Targeted Killing of Staphylococcus aureus on ´the Race for Surface´ Colonization 
  • This dataset was generated through experimental work focused on the use of thiolated hyaluronic acid (HAMS)  as a switch for the targeted delivery of polyphosphate nano encapsulated peptidoglycan hydrolase M23 (M23-PP)

Technical details

  • For naming the samples comprising M23-PP coated with HAMS, a logical system was applied and was maintained across all the publications related with this dataset. 
  • This dataset can be opened using Microsoft Excel, a part of the Microsoft Office suite
  • This dataset was published and is associated to a DOI

Further details

  • For further reference, please read Thiolated Hyaluronic Acid: A Gateway for Targeted Killing of Staphylococcus aureus on ´the Race for Surface´ Colonization 

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Additional details

Related works

Is part of
Dataset: 10.1002/adhm.202502890 (DOI)

Funding

European Commission
EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions NanoBioRS-101025065

Dates

Available
2025-09-12